Figure 5. Effect of pertussis toxin on the expression of cell cycle components from G1-phase in vascular smooth muscle cells (VSMC) from SHR and WKY rats.

VSMC from SHR and WKY rats were incubated for 16 h in the absence (control) or presence of pertussis toxin (PT, 10 ng/ml). The cell lysates were used for Western blotting as described in “Methods”. Results are expressed as % of WKY CTL, taken as 100%. Values are means ± SE of 5 separate experiments. *P<0.05, **P<0.01 ***P<0.001.