Figure 8. Effect of wortmannin on expression of cell cycle components from G1-phase in vascular smooth muscle cells (VSMC) from SHR and WKY rats.

VSMC from SHR and WKY rats were incubated for 16 h in the absence (control) or presence of 1 µM wortmannin WM). The cell lysates were prepared and used for Western blotting using specific antibodies against cyclin D1 (A), cdk4 (B), cdk2 (C) and pRB (D) as described in “Methods”. Results are expressed as % of WKY CTL taken as 100%. Values are means ± SE of 5 separate experiments. *P<0.05, **P<0.01, * **P<0.001.