Figure 2. NHBE cells express CXCR4 but not CCR5 and HIV gp120 induces mucus through CXCR4.
(A): NHBE cell RNA was analyzed by qPCR for the expression of CXCR4 and CCR5 up to 40 cycles of amplification. (B): gp120LAV-induced mucus formation was determined in the presence or absence of CXCR4 inhibitor AMD3100 and CCR5 inhibitor maraviroc. (C): gp120-induced mRNA expression of MUC5AC and GABAARα2 was determined in the presence and absence of AMD3100 and maraviroc by qPCR analysis. Each experiment was repeated at least three times; bars represent mean ± SEM.