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. 2013 Oct 14;8(10):e77060. doi: 10.1371/journal.pone.0077060

Figure 4. Effect of ACSL4 expression on invasion capability of breast cancer cells.

Figure 4

MCF-7 cells were stably transfected with ACSL4 cDNA utilizing a lentivirus vector, and control- and ACSL4-tranfected cells compared with respect to invasive potential as described in the text. Panel A, control MCF-7, B, ACSL4-MCF-7, C, bar graph indicating the average number of cells per field in 3 separate chambers (p=0.005), and an immunoblot blot analysis of ACSL4 expression in control and ACSL4- MCF-7 cells. For panels D, E and F, MDA-MB-231 were treated with either control or ACSL4 siRNA (20nM), and control and experimental cells were compared with respect to invasive potential as described in the text. Panel D, control siRNA-treated cells, E, ACSL4 siRNA-treated cells, F, bar graph indicating the average number of cells per field in 3 separate chambers (p=0.005), and an immunoblot blot analysis of ACSL4 expression in control siRNA and ACSL4 siRNA-treated cells.