Figure 4. Identifying the Cdc48-binding domain of Irc20.

A) Diagram of Irc20 deletion derivatives and a summary of their ability to co-IP Cdc48. B) & C) Co-immunoprecipitation analysis. The indicated Irc20 derivatives were expressed from a 2 μ plasmid in the presence of Cdc48–3xMyc, extracts were prepared, proteins immunoprecipitated with anti-HA beads, and western blotted with anti-HA antibody to detect Irc20, or with anti-Myc antibody to detect Cdc48. The binding interface of Irc20 that physically interacts with Cdc48 lies between amino acids 883 and 1238.