Table 1. Yeast two-hybrid assay.
DNA binding fusion | Transcriptional activation fusion | Units of β-galactosidase |
---|---|---|
FOXP3ΔFKH | clone (LCK) | 3.08 |
FOXP3ΔFKH | Non-reactive clone | 0.34 |
Positive control | 10.0 | |
Negative control | 0.53 |
One of the positive clones resulting from screening with Foxp3 ∆FKH (1-332 amino acids) as a bait was identified as encoding a portion of LCK.