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. 2013 Oct 14;8(10):e76937. doi: 10.1371/journal.pone.0076937

Table 2. Comparison of the estimated operational taxonomic unit (OTU) richness and diversity indexes of the 16S rRNA gene libraries for clustering at 97% identity as obtained from the pyrosequencing analysis.

Manure ratio	Reads	OTU	ACE	Chao	Coverage	Shannon	Simpson
0%	9015±2997^a	3533±812^a	9581±2336^a	6816±1559^a	0.767±0.024^a	7.58±0.112^a	0.0010±5.7735^b
5%	9485±2305^a	4032±878^a	1101±2941^a	7754±1732^a	0.749±0.005^a	7.776±0.179^a	0.0007±5.7735^a
10%	8501±1153^a	3643±478^a	9814±1751^a	7204±1217^a	0.747±0.026^a	7.706±0.117^a	0.0008±5.7735^a
15%	9138±1096^a	3871±213^a	1069±835^a	7648±393^a	0.749±0.019^a	7.766±0.005^a	0.0007±0.0001^a
20%	9916±1429^a	4136±496^a	1105±1744^a	8133±1134^a	0.755±0.032^a	7.836±0.116^a	0.0007±0.0001^a
25%	7991±768^a	3354±393^a	9651±2464^a	6903±1290^a	0.749±0.019^a	7.573±0.081^a	0.0010±5.7735^b

Averages of replicates ± standard error; means followed by different letters are significantly different at P<0.05.