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| Sequence 5′–>3′ | annealing temp | amplicon length | |
| ESR1_rs9340799_XbaI_F | CTGCCACCCTATCTGTATCTTTTCCTATTCTCC | 71°C | 1300 bp |
| ESR1_rs9340799_XbaI_R | TCTTTCTCTGCCACCCTGGCGTCGATTATCTGA | ||
| ESR1_rs2234693_PuvII_F | AGGCTGGGCTCAAACTACAG | 60°C | 759 bp |
| ESR1_rs2234693_PuvII_R | TCCTTGGCAGATTCCATAGC |
