Fig. 8.

Cell morphology with PDMS post microtextures that are 10 µm diameter (D), 10 µm and 40 µm inter-space (IS) between the posts with the 10 µm and 40 µm heights (H). (a) CTPs on 10 µm height of post microtextures with 10 µm inter-spaces interact with the bottom of the microtextures as well as with the sides. Cells tended to attach next to the posts and spread between and top of them, exhibited highly contoured morphology, and directed their long processes towards posts and other cells. (b) On the 10 µm height micropost with 40 µm inter-spaces, cells are likely to have contact to only one micropost side and the bottom at the same time. The morphology of cells grown on the microposts showed to anchor to random locations and cell bodies adopted a broad flattened shape. (c) Cells on post microtextures with 40 µm heights and 10 µm inter-spaces attach and spread on the top of the them, and pulled posts around cells. (d) Cell morphology on post microtextures with 40 µm heights and 40 µm inter-spaces bent posts around cells and exhibited similar shape to cells on smooth surfaces that appeared to anchor to random locations on the surface as they migrated and cell bodies adopted a broad flattened shape.