Table 1.
New miRNA | Query miRNA | GenBank acc. No. | Tissue type | Arm | SP | EP | ME | Mature sequence* | E value | PL | A+U (%) | MFE (kcal/mol) | MFEI |
miR156 | aly-miR157a-5p | HS396956.1 | Leaf | 5′ | 212 | 232 | 20/21 | UUGACAGAAGAUAGAGAGCAu | 1.00E−04 | 100 | 54.00 | 45.80 | 1.00 |
miR171a | ptc-miR171k | FS948108.1 | Roots | 3′ | 273 | 293 | 20/21 | GGAUUGAGCCGCGCCAAUAUu | 1.00E−04 | 100 | 65.00 | 39.20 | 1.12 |
miR171b | ptc-miR171k | FS948109.1 | Roots | 5′ | 163 | 143 | 20/21 | GGAUUGAGCCGCGCCAAUAUu | 1.00E−04 | 97 | 63.92 | 40.40 | 1.15 |
miR397 | osa-miR397b | CV699725.1 | Leaf | 5′ | 99 | 79 | 21/21 | UUAUUGAGUGCAGCGUUGAUG | 3.00E−05 | 126 | 59.52 | 39.20 | 0.77 |
miR399 | osa-miR399j | FS958856.1 | Young leaves | 3′ | 109 | 129 | 20/21 | UGCCAAaGGAGAGUUGCCCUA | 8.00E−03 | 103 | 51.46 | 52.80 | 1.06 |
miR2863 | osa-miR2863a | FS950435.1 | Roots | 5’ | 37 | 57 | 18/21 | UauaUAUUGUUGAAAUGGCUU | 8.00E−03 | 85 | 64.71 | 21.50 | 0.72 |
miR2911a | nta-miR2911 | JK476023.1 | Leaf | 3′ | 364 | 383 | 20/20 | GGCCGGGGGACGGACUGGGA | 1.00E−04 | 97 | 22.68 | 65.40 | 0.87 |
miR2911b | nta-miR2911 | FS953337.1 | Shoot stems | 3′ | 177 | 196 | 20/20 | GGCCGGGGGACGGACUGGGA | 1.00E−04 | 97 | 23.71 | 69.20 | 0.94 |
miR5021a | ath-miR5021 | GW690847.1 | Bud | 3′ | 256 | 237 | 18/20 | aGAGAAGAAGAAGAAGAAAg | 2.00E−03 | 195 | 55.90 | 71.90 | 0.84 |
miR5021b | ath-miR5021 | GE651759.1 | Tender root | 5′ | 73 | 92 | 18/20 | aGAGAAGAAGAAGAAGAAAg | 2.00E−03 | 201 | 56.22 | 72.20 | 0.82 |
miR5368a | gma-miR5368 | GE653011.1 | Tender root | 3' | 505 | 523 | 19/19 | GGACAGUCUCAGGUAGACA | 4.00E-04 | 163 | 42.33 | 76.10 | 0.81 |
miR5368b | gma-miR5368 | FS945766.1 | Mature leaves | 3′ | 196 | 214 | 19/19 | GGACAGUCUCAGGUAGACA | 4.00E-04 | 144 | 42.36 | 68.50 | 0.83 |
miR6483a | hbr-miR6483 | HS398296.1 | Leaf | 5′ | 253 | 232 | 21/22 | UAUUGUAGAAAUUUUCgGGAUC | 2.00E−03 | 101 | 62.38 | 29.80 | 0.78 |
miR6483b | hbr-miR6483 | JK714410.1 | Leaf | 5′ | 303 | 282 | 21/22 | UAUUGUAGAAAUUUUCgGGAUC | 2.00E−03 | 110 | 63.64 | 30.00 | 0.75 |
Lowercase letters in mature sequence mean mismatch
SP: start point; EP: end point; ME: match extent; PL: pre-miRNA length; MFE: minimal folding free energy; MFEI: MFE index