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. 2013 Oct 15;3:2938. doi: 10.1038/srep02938

Figure 3. DSS-only and AOM-only models of sporadic colon carcinogenesis.

Figure 3

(a) Top, AOM (10 mg/kg) was weekly injected during six weeks. Bottom, fresh 2.5% DSS was given in drinking water during five-day cycles (gray areas) followed by regular drinking water for 16 days. Blood samples were collected at the end of the protocols and also for AOM-treated mice at day 63. (b) Representative images of distal colon macroscopic appearance of DSS-treated (day 63) and AOM-treated mice at day 63 and 250. Tumors are highlighted with black arrows. (c) Representative haematoxylin/eosin staining sections of distal colon morphology of DSS- and AOM-treated mice and control mice. No significant changes were observed for AOM-treated mouse at day 63. DSS-treated mice showed presence of adenomas and different grade of dysplasia (DSS 1, DSS 2 and DSS 3) at day 63, whereas DSS 4 animal showed low grade nonmalignant dysplastic lesions and DSS 5 showed no changes respect to normal mucosa. (d) At day 250, three AOM-treated mice showed infiltrating but non-invasive tumors (AOM 2, AOM 3 and AOM 5), whereas the remaining animals (AOM 1 and AOM 4) showed small flat non-polipoid adenomas with dysplasia. (c, d) Black arrows highlight the observed lesions. Images are shown at 200x magnification.