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. 2013 Oct 15;3:2941. doi: 10.1038/srep02941

Figure 1.

Figure 1

In vitro transglycation activity of hydralazine (A) Glycated insulin (m/z 5970) was incubated with either 0 mM, 25 mM or 50 mM hydralazine for three hours at 37°C and formation of unglycated insulin (m/z 5808) was monitored by MALDI-TOF-MS. Hydralazine showed concentration dependent transglycation of glycated Insulin. (B) MALDI-TOF-MS analysis of hydralazine-glucose conjugate formed during transglycation assay where m/z 161.08 and m/z 323.13Da are hydralazine and hydralazine-glucose conjugate respectively.