Fig. 2.
Ionomycin treatment induces gravin redistribution. (A) Fluorescent micrographs illustrating that gravin-EGFP undergoes redistribution immediately following the addition of 1 μM ionomycin. (B) Plot of membrane:cytosol fluorescence intensity ratio over time illustrating the time course of gravin redistribution. (C) Corresponding plot of the Fura2 fluorescence ratio illustrating the rapid increase in [Ca2+]i upon ionomycin treatment. Note that gravin translocation is complete at t = 60 seconds, which corresponds to the time at which maximal cytosolic calcium increase is observed.