Skip to main content
. Author manuscript; available in PMC: 2014 Nov 1.
Published in final edited form as: Cell Signal. 2013 Jul 6;25(11):2125–2135. doi: 10.1016/j.cellsig.2013.07.004

Fig. 7.

Fig. 7

Regulation of gravin redistribution by ATP treatment. (A-J) Fluorescence micrographs illustrating the effect of ATP treatment (10 mM) on gravin distribution in the presence of bisindolylmaleimide (BIM), BAPTA-AM, or BIM and BAPTA-AM together. ATP treatment induced complete redistribution of gravin (C,D) compared to untreated cells (A,B). Gravin redistribution was partially attenuated when ATP was administered in the presence of 2 μM bisindolylmaleimide (BIM) or 50 μM BAPTA-AM (G,H). However, gravin redistribution was fully prevented when ATP was administered in the presence of both BIM and BAPTA-AM (I,J). (K-M) Quantitative analysis of gravin-EGFP dynamics in response to ATP in the presence of BIM and BAPTA-AM. BIM (K) or BAPTA-AM (L) caused a significant attenuation in the redistribution of gravin-EGFP. However, ATP treatment in the presence of both BIM and BAPTA-AM completely inhibited redistribution of gravin-EGFP (M). (N) Histogram illustrating the extent to which gravin distribution changed under the different treatment conditions. The height of the bars corresponds to the difference between the membrane/cytosol ratio at t=0 and the minimum membrane/cytosol ratio reached during each run. One-way ANOVA followed by Holm-Sidak post hoc tests revealed three significantly distinct responses to the treatments, which are denoted by asterisks (p < 0.05). Scale bar = 10 μM.