Fig. 6.

SPECT/CT imaging of ¹²³I accumulation in М3 melanoma tumor after NIS gene transfer. Reconstructed 3D-projections of mice at 24 h after injection of targeted polyplexes with plasmid DNA encoding NIS (left) or targeted polyplexes containing plasmid without promoter as a control (right) (a). Reconstructed images of saggital planes passing through the tumors of five mice at 24 h after injection of targeted polyplexes (b). NIS expression with time measured by iodide uptake after intravenous administration of targeted polyplexes, containing 80 μg of plasmid DNA encoding NIS (c). ¹²³I accumulation in tumor at 24 h after injection of targeted (PEI-PEG-MC1SP/DNA) polyplexes compared to non-targeted (PEI-PEG/DNA) ones and targeted polyplexes containing plasmid without promoter (control polyplexes) (d). Background level observed in non-transfected control (2.4 ± 0.4 % ID/g) was subtracted in (d). 18.5 МBq of ¹²³I was injected intravenously for visualizing of NIS gene expression. All values are means ± SD % ID/g. *p < 0.001, **p < 0.0001.