Figure 6.

Unique sperm lnc-RNAs isoforms. A 30 kb region of chromosome 3 containing a series of putative lnc-RNAs as identified by the Human Body Map lincRNA UCSC track (Trapnell et al., 2010; Cabili et al., 2011) is shown in upper panel. Although low- level expression of a number of identified lnc-RNAs is evident across this region in testes, a single highly expressed two exon RNA is observed in sperm (lower panel). Many junction reads, as measured by RUM (Grant et al., 2011) (box), confirm that these two exons are part of a single spliced transcript, which was not previously identified as a unique lnc-RNA isoform. (See Supplementary data, Fig. S3 for more details.)