Table 1. Sequence and three-isotope codes of the 109 peptides tested in the rotavirus epitope screen.
| Rotavirus epitopes | Metal code | Rotavirus epitopes | Metal code | Previously characterized epitopes | Metal code | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
|
|
|
|
|
|
|
|||||||||
| Epitope | Sequence | 1 | 2 | 3 | Epitope | Sequence | 1 | 2 | 3 | Epitope | Sequence | 1 | 2 | 3 |
| NSP1_01 | KMYEHIEDV | 154 | 163 | 173 | VP2_21 | FLAGINSQA | 157 | 161 | 163 | CMV_01 | NLVPMVATV | 154 | 155 | 157 |
| NSP1_02 | FLDSEPHLL | 154 | 163 | 169 | VP2_22 | RLLSYNYET | 155 | 159 | 161 | CMV_02 | VLAELVKQI | 159 | 165 | 169 |
| NSP1_04 | FLDSEPHL | 154 | 169 | 175 | VP2_28 | FVTDSSVISL | 155 | 163 | 169 | CMV_03 | VLEETSVML | 159 | 169 | 175 |
| NSP1_06 | FLDSEPHLLRM | 154 | 157 | 163 | VP2_36 | FVSADTVEPI | 155 | 161 | 169 | EBV_01 | YVLDHLIVV | 159 | 163 | 175 |
| NSP1_07 | VLFNHEVNWDV | 154 | 161 | 165 | VP3_01 | YMLSDNTYV | 157 | 159 | 161 | EBV_02 | GLCTLVAML | 161 | 169 | 175 |
| NSP1_09 | KMYEHIEDVL | 154 | 163 | 175 | VP3_03 | YLHDYSYYT | 157 | 163 | 173 | EBV_03 | YLLEMLWRL | 154 | 155 | 161 |
| NSP2_01 | YLDHKLTPI | 155 | 157 | 165 | VP3_04 | YMLGSAPSYWI | 159 | 163 | 165 | EBV_04 | CLGGLLTMV | 161 | 165 | 173 |
| NSP2_02 | FTMWKLTYL | 154 | 173 | 175 | VP3_05 | FLISNLTTHNI | 157 | 169 | 175 | EBV_05 | YLQQNWWTL | 154 | 155 | 163 |
| NSP2_03 | YLDHKLTPIL | 154 | 157 | 169 | VP3_06 | YMLSDNTYVA | 155 | 159 | 163 | EZH2 | YMCSFLFNL | 154 | 165 | 175 |
| NSP2_04 | KMDEVSHVGV | 154 | 161 | 169 | VP3_07 | KLFPSIVNI | 155 | 161 | 173 | FluM1 | GILGFVFTL | 154 | 155 | 159 |
| NSP2_05 | KMDEVSHV | 154 | 159 | 173 | VP3_08 | FLPTDFEL | 157 | 159 | 163 | FluNP | KLGEFYNQMM | 154 | 155 | 169 |
| NSP2_10 | RIIWQNWYA | 155 | 157 | 159 | VP3_09 | YLLPGWKLTYV | 155 | 163 | 173 | GAD | VMNILLQYL | 159 | 163 | 173 |
| NSP5_01 | YMLSKSPEDI | 155 | 159 | 169 | VP3_11 | MLSDNTYVA | 157 | 163 | 175 | HBV_01 | FLLTRILTI | 163 | 165 | 169 |
| NSP5_05 | SLPSISSSI | 155 | 165 | 169 | VP3_13 | KLYNMFYRNYI | 155 | 159 | 165 | HBV_02 | WLSLLVPFV | 159 | 173 | 175 |
| VP1_01 | KIYSWSFHV | 159 | 161 | 163 | VP3_14 | YTYMLSDNTYV | 157 | 161 | 165 | HBV_03 | FLPSDFFPSV | 165 | 169 | 175 |
| VP1_02 | FILPYEYFI | 155 | 157 | 169 | VP3_15 | KLLHHPTTEV | 157 | 159 | 165 | HBV_04 | GLSPTVWLSV | 159 | 165 | 173 |
| VP1_03 | ILSEYLSFV | 155 | 161 | 175 | VP3_17 | FISDNMIHDV | 157 | 165 | 169 | HBV_05 | FLLSLGIHL | 163 | 169 | 173 |
| VP1_05 | LMDPAILTSL | 155 | 159 | 173 | VP3_18 | YLLPGWKL | 157 | 173 | 175 | HCV | ALYDVVTKL | 154 | 155 | 173 |
| VP1_06 | SLMDPAILTSL | 155 | 165 | 173 | VP3_19 | LLPGWKLTYV | 155 | 163 | 175 | HIV_01 | NVWATHACV | 161 | 173 | 175 |
| VP1_07 | KMWNITAL | 159 | 161 | 165 | VP6_01 | NIFPYSASFTL | 154 | 163 | 165 | HIV_02 | TLNAWVKVV | 169 | 173 | 175 |
| VP1_08 | KLWKKMWNI | 157 | 159 | 169 | VP6_02 | FLLNGQIINT | 154 | 169 | 173 | HIV_03 | FLGKIWPS | 161 | 165 | 175 |
| VP1_12 | YLVTWANSSI | 157 | 165 | 173 | VP6_03 | VLADANETL | 154 | 161 | 163 | HIV_04 | KLTPLCVTL | 163 | 165 | 175 |
| VP1_13 | NLMDSYVQI | 157 | 161 | 169 | VP6_04 | TLLANVTAV | 154 | 157 | 173 | HIV_05 | SLYNTVATL | 165 | 169 | 173 |
| VP1_14 | KLNSYAPVYL | 157 | 161 | 175 | VP6_05 | LLANVTAV | 154 | 161 | 175 | HIV_06 | LTFGWCFKL | 161 | 165 | 169 |
| VP1_20 | YLIAKELIIL | 159 | 161 | 169 | VP6_06 | GLLGTTLLNL | 154 | 159 | 163 | HIV_07 | GLADQLIHL | 161 | 169 | 173 |
| VP1_22 | FILPYEYFIA | 155 | 159 | 175 | VP6_07 | TLLNLDANYV | 154 | 159 | 175 | HIV_08 | ALVEMGHHA | 159 | 165 | 175 |
| VP1_26 | SLMDPAIL | 155 | 165 | 175 | VP7_01 | LLNYILKSV | 154 | 159 | 169 | HIV_09 | ILKEPVHGV | 163 | 173 | 175 |
| VP1_27 | NLNAVMFWL | 157 | 159 | 173 | VP7_02 | FVIYRFLFV | 154 | 161 | 173 | HPV | YMLDLQPETT | 154 | 157 | 161 |
| VP1_32 | FMPTLPDNV | 155 | 169 | 175 | VP7_03 | VIYRFLFVI | 154 | 159 | 161 | HSV1/2 | SLPITVYYA | 154 | 155 | 175 |
| VP1_39 | ILESYVYNL | 155 | 157 | 173 | VP7_04 | YTDVASFSV | 155 | 157 | 163 | LCMV | YLVSIFLHL | 154 | 165 | 169 |
| VP2_01 | MLTSNLTFTV | 157 | 165 | 175 | Unused metal codes (negative controls) | Malaria | YLNKIQNSL | 161 | 163 | 169 | ||||
| VP2_02 | ALVGALPFV | 157 | 159 | 175 | UNUSED | 161 | 163 | 175 | Mart1 | ELAGIGILTV | 154 | 155 | 165 | |
| VP2_03 | SLISGMWLLTV | 155 | 157 | 175 | UNUSED | 165 | 173 | 175 | MV | KLWCRHFCV | 154 | 157 | 159 | |
| VP2_04 | SLISGMWLL | 159 | 161 | 173 | UNUSED | 154 | 157 | 165 | RSV | KMLKEMGEV | 159 | 163 | 169 | |
| VP2_05 | HMLTSNLTFTV | 155 | 161 | 163 | UNUSED | 154 | 157 | 175 | TB_01 | GLPVEYLQV | 163 | 169 | 175 | |
| VP2_06 | QLMEALMQL | 155 | 161 | 165 | UNUSED | 154 | 165 | 173 | TB_02 | KLIANNTRV | 163 | 165 | 173 | |
| VP2_07 | FLNHQLVEPL | 157 | 169 | 173 | UNUSED | 155 | 169 | 173 | Tyr-ase | YMDGTMSQV | 155 | 157 | 161 | |
| VP2_10 | TLFHYYNVNV | 157 | 163 | 165 | UNUSED | 157 | 161 | 173 | ||||||
| VP2_11 | YMSLISGMWLL | 155 | 173 | 175 | UNUSED | 159 | 169 | 173 | ||||||
| VP2_12 | MLLNNQPVALV | 157 | 163 | 169 | UNUSED | 161 | 163 | 173 | ||||||
| VP2_16 | MLSQRTMSL | 159 | 161 | 175 | UNUSED | 161 | 169 | 175 | ||||||
| VP2_17 | RLLSYNYETL | 155 | 163 | 165 | UNUSED | 154 | 159 | 169 | ||||||
With 120 codes possible using ten metals, 11 codes act as internal negative controls (labeled ‘unused’). Metals associated with each isotope are listed in Supplementary Table 1. MV, measles virus; HPV, human papilloma virus; HSV1/2, herpes simplex virus; TB, tuberculosis; LCMV, lymphocytic choriomeningitis virus.