Pravastatin interfered with BKV entry into HRPTEC. (upper) HRPTEC were incubated with BKV (MOI=5 FFU/cell) alone for 6, 12, 24, and 48 hr. (lower) HRPTEC were preincubated with 50 μM of pravastatin for 1 hr before another 6, 12, 24, and 48 hr co-incubation with labeled BKV (MOI=5 FFU/cell) and 50 μM of pravastatin. After incubation, Alexa Fluor 488 labeled BKV particles were observed by confocal microscope with 63× objective lens. Cross-section confocal images of the interior of cells incubated with labeled BKV are shown for each incubation time (right). Horizontal and vertical lines indicate the X and Y planes through which the cross-sectioned images were made. Bars are 25 μM (left) and 10 μM (right).