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The effect of pravastatin after BKV infection was established. HRPTEC were incubated with BKV (MOI=0.5 FFU/cell) for 72 hr. Medium was removed and cells were incubated for another 48 hr with pravastatin (25 and 50 μM). After 5 days incubation, cells were analyzed by indirect immunofluorescent analysis (magnification ×20). (upper) T-Ag positive cells were counted as BKV infected cells and the percentage was calculated against DAPI stained nuclei (lower) as total cells.
