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. 2013 Oct 15;8(10):e77383. doi: 10.1371/journal.pone.0077383

Figure 3. Expression of hspB1 protein and mRNA is controlled by the cell cycle.

Figure 3

A, MEF were synchronized by serum starvation for 48% FCS-containing medium and analysed by western blot for hspB1, PCNA, p27kip1 and actin. B, Comparison of the expression of mRNAs for hspB1 and the cell cycle-regulated genes, Myc, and Cyclin E1 determined by qRT-PCR and normalized to GAPDH in a representative synchronized MEF serum release time course. C, Western blot for hspB1, PCNA and loading control, actin, in lysates of MEF following release from nocodazole G2/M block (40 ng/ml). All western blots shown are representative of at least two independent experiments.