Figure 5. HspB1 protein expression in unwounded and wounded murine skin.

A, Unwounded female wild-type skin was stained with Masson’s trichrome; (bar = 100 µm). B, HspB1 was detected by IHC in unwounded female wild-type. Inset: High power image showing hspB1 staining in cells with fibroblast-like morphology in connective tissue beneath panniculus carnosus. C, Unwounded female hspB1 ^del/del skin showing lack of staining with anti-hspB1 antibody. Inset as for (B); (bar = 10 µm). D, HspB1 staining at d3 post-wounding showing expression in epithelial tongues and cells in granulation tissue (representative wounds from 11 mice in three experiments) E, High power image of boxed region indicated in (D) showing hspB1 expressing cells with fibroblast-like morphology in granulation tissue. F, hspB1 staining at d7 showing expression in newly formed muscle, and epithelium (representative wounds from 7–8 mice in two experiments); (bar = 500 µm). G, High power image of boxed region indicated in (F) showing expression of hspB1 in newly formed skeletal muscle and microvasculature; (bar = 10 µm). 11–14 week age-matched female wild-type mice were used.