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. 2013 Oct 15;8(10):e77383. doi: 10.1371/journal.pone.0077383

Figure 9. HspB1 deficiency increases neutrophil infiltration and chemokine expression in the zymosan-induced air-pouch and peritonitis models of acute inflammation.

Figure 9

A, Air-pouches were created on the dorsal surfaces of 10–12 week old male wild-type (closed circles) hspB1 del/del (open circles) mice and were injected with 100 µl of 1 mg/ml zymosan. Exudates were retrieved at the indicated times. Plot of number of trypan blue-negative infiltrating cells counted with a haemocytometer (n = 5–6 mice per group for 2 h post-zymosan; n = 9–14 from two experiments each for all other times). Plots of CXCL-1, and CXCL-2 protein in exudate supernatants measured by ELISA at 1 h post-zymosan (n = 9 from two experiments). Graphs show mean±SEM. B, 1 ml of zymosan (1 mg/ml) was injected into the peritoneal cavities of 10–12 week-old male wild-type and hspB1 del/del mice. Plot of number of infiltrating cells with time of zymosan treatment indicated (n = 4–6) and CXCL-1 and CXCL-2 protein in exudates at as for (A) (n = 10–12 from two experiments); *P<0.05, **P<0.01, ***P<0.001.