Figure 1. Titration analysis of live GC cells used to immunize mice and bound to mouse serum.

Human PBMCs were isolated from whole blood of a selected healthy donor for normal cell control. MKN45, BGC823, SGC7901, and MKN28 cells were used in experiments, but SGC7901 and BGC823 cell lines were randomly selected as examples in this figure. The MFI were higher in these cells than in PBMCs. All three mice exhibited strong immune responses to the human GC cell lines.