Table 1. Microsatellite loci assembled in two multiplex PCR sets, and used to genotype Tetranychus urticae mites (details on microsatellite isolation and characterization are described in [35,36], see Information S2).

Locus	Core repeat	Size of cloned allele (bp)	Size range (bp)	Fluorescent label	Primer (µM)	Primer sequence (5'-3')
PCR multiplex set 1 (Annealing temperature: 58°C)
TuCA72	(GT)6	262	264-270	VIC	0.02	F: ACTGTCTGGCGTCTTTTGTC
						R: GTTTACCAGTTTCCCTTTGACC
TuCT37	(TC)7C(TC)5	128	121-127	VIC	0.02	F: GTAATAATGGGTGTTGTTGC
						R: CGCAAAATATGAGTGAGAATG
Tu11	(GT)29	154	148-168	PET	0.02	F: CGTGTACAATCAGTCAACATCC
						R: TGGACTTTTTAACCGTGGCT
Tu27	(GA)9	66	57-84	PET	0.02	F: TCAAACTTGCTTTTTTCAC
						R: GTGATGAAAATGGAGATGG
TuCA12	(CA)7	276	263-275	FAM	0.02	F: GATTTGTGGTCGTGGTTTTC
						R: GATCAACTCAAAAGGATAACGTTG
TuCT09	(CT)15	129	102-128	FAM	0.02	F: GATCACTTTTTCATGTTATTCTG
						R: CTTGGAATGAACTTTAGCAC
TuCA25	(TC)13	164	155-165	FAM	0.02	F: AATGTGTTGGTTGTTTACGAAGTG
						R: TTGGTCAAAGCCGGTTACAG
TuCT13	(TC)7	164	163-168	NED	0.02	F: CAGATGGATTTGCTTTCCAC
						R: GATCCTAATCAACATGAGGGTC
TuCT81	(TC)9	346	345-350	NED	0.02	F: GATTCGTGAAGCCGATATTGA
						R: GTCATCATCGTTACAAATTCTG
TuCT67	(CT)9	95	95-107	NED	0.02	F: CCATCATCTTCATCATTCTTCACC
						R: TAGAACAGTCAAGCAAAAAGAGTC
PCR multiplex set 2 (Annealing temperature: 52°C)
TuCT73	(CA)7	111	111-113	VIC	0.02	F: CGATGTGGGTGGTAAGCATG
						R: ACGATGATATTGATGATGAGCG
TuCA83	(GT)6	205	206-208	VIC	0.02	F: CAGGGTGAAACTTAGATACC
						R: CAATTTTCCCTCTACATCTC
Tu16	(GAT)7	136	130-148	PET	0.02	F: TTCCAATGGAAAGTGGATTTG
						R: TCAATACCAAACGAAACTGGC
Tu1	(GT)30	152	144-182	PET	0.02	F: GATGTAAAGGAGCGCAAAGG
						R: CATTGTTTGTCGATTTCCTCTC
TuCT17	(GA)17	318	288-320	NED	0.02	F: GTGATTGTTTGGCGTGCTTAG
						R: CATTGACCAGAGCGACATTG
TuCT26	(GA)8	154	150-156	NED	0.02	F: CGATGGAGCCGTTTCAAGAG
						R: TCGTCATCATTGCCGTCATTTTAC
Tu35b	(TGA)8	108	99-120	NED	0.02	F: AATGGAATGAGTTATCGTTGGG
						R: CTTCCCGAAGGCTGTTGATA
TuCT18	(CT)9	296	294-296	FAM	0.02	F: CTTGATGCTAGTGATACAACG
						R: CAAGGTGATGATTTGATTTAAAG
TuCT04	(CT)8	149	146-152	FAM	0.02	F: CGTCATCATTGCCGTCATTTTAC
						R: GGAGCCGTTTCAAGAGAGTG
TuCA96	(TG)7	111	107-109	FAM	0.02	F: ATGGATTGTCACCGATTTCA
						R: CTGAAGTTTACTTGCTATAGTC