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. 2013 Oct 15;8(10):e77299. doi: 10.1371/journal.pone.0077299

Figure 4. NOTCH3 knockdown alters cell cycle progression and induces apoptosis via downregulation of Cyclin D1 and EGFR.

Figure 4

(A) Cell cycle profile of U87-MG cells on day 5 post-transduction with shRNA or Mock virus. Cells were synchronized to the G1/S boundary with serum free media and treated with sh235. Cell cycle was assessed by propidium iodide staining followed by FACS analysis. Results are shown in histogram distribution of the 3 major cell cycle steps. (B) NOTCH3 knockdown induced apoptosic DNA fragmentation in U87-MG cells. Extracted DNA was analyzed by DNA integrity on agarose gel. Visible DNA ladder in U87-MG cells following NOTCH3 knockdown is shown. (C) Immunoblot analysis of potential NOTCH3 targets protein content following NOTCH3 knockdown.