Skip to main content
. 2013 Oct 15;8(10):e77118. doi: 10.1371/journal.pone.0077118

Figure 2. SSA-Agarose column choromatography performed in the 4 patients with type 2 diabetes.

Figure 2

A. The concentrated urine samples were applied to SSA-Agarose column, washed with PBS and eluted with 0.2 M lactose. B. The effluents from the patients manifested with various albuminria and GFR stages, A3G3 and A3G4, were subjected to SDS-PAGE and stained with Coomassie Brilliant Blue. The bands were visualized and they were subjected to liquid chromatography-tandem mass spectrometer (LC/MS-MS) analysis.