Table 4. Summary of immunolocalization experiments for membrane transporter proteins in vaginal tissue specimens from the 6 subjects spanning multiple locations.
|
Protein (Gene)
|
|||||||||
|---|---|---|---|---|---|---|---|---|---|
| Subject ID, Sample Location | BCRP (ABCG2) | MDR1 (ABCB1) | MRP1 (ABCC1) | MRP2 (ABCB2) | MRP3 (ABCB3) | MRP4 (ABCB4) | OAT1 (SLCA6) | OAT3 (SLCA8) | Sec |
| 5001, Ant-Int | Musc + wk | - | Endo + | Endo + | EC + str | EC + | Musc/Endo + | Endo + | - |
| 5001, Post-Int | Musc/EC + | - | Endo + | Musc/Endo + | EC + str | - | Musc + | EC + | - |
| 5001, Post-Ext | Musc/Endo + | EC/Progen + | Endo + | Musc/Endo + | EC + str | Musc/Endo + | EC/Progen + | EC/Endo + | - |
| 5002, Post-Int | Musc + wk | - | Endo + | Endo + | EC + | - | Musc/EC + | Endo + wk | - |
| 5003, Post-Int | Musc + | - | Endo + | Ap EC/ Endo + | EC + str | Musc/Endo + | + wk | EC/Endo + wk | - |
| 5004, Post-Int | Musc + | Fibro/Endo + | Endo + | Endo + | EC + str | Musc/Endo + | Musc + wk | Musc/Endo + wk | - |
| 5005, Post-Int | Musc + wk | Fibro/Endo + wk | - | Musc/Endo + | EC + wk | Musc/Endo + | Musc + wk | Musc + wk | - |
| 5006, Post-Int | Musc + | Fibro/Endo + | - | Musc/Endo + | EC + str | Endo + wk | Musc + wk | Musc + wk | - |
Data represent results from at least 5 distinct 8 µm sections and at least 5 low (10×) and high (40×) magnification fields. Tissue collection locations (see Figure 1): Ant, anterior; Post, posterior; Int, interior; Ext, exterior. Cell types where membrane transporters were localized: Musc, muscular tissue; EC, epithelial cells; Endo, endothelial cells; Progen, progenitor epithelial cells; Fibro, fibroblast; + membrane transporter protein consistently observed by immunofluorescence microscopy; - membrane transporter protein not observed by immunofluorescence microscopy; str, strong signal/expression; wk, weak signal/expression.