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. 2013 Oct 15;8(10):e77286. doi: 10.1371/journal.pone.0077286

Figure 3. Role of IL-1R1/IL-1 signaling in mediating Pax6 loss and altered lineage commitment in autoimmune-mediated KCS/SQM.

Figure 3

(A) Adoptive transfer (AT) experimental design. (B) Transcriptional profile of corneolimbal epithelial Pax6 in the four AT groups with control group (WT scid) set as the reference (designated 1-fold) to generate relative quantitation (RQ) values of Pax6 expression. Data are shown as mean RQ value ± SE; statistically significant differences are noted as P<0.01 (**); n=5 per group. (C) Immunolocalization studies of Pax6 staining in the four AT groups (red); Scale bar = 50μm. (D) IL-1β and Pax6 transcript levels in WT and Aire KO mice expressed as mean ± SE RQ values where an arbitrary WT control mouse was set as the reference (designated 1-fold); P<0.05 (*) indicates statistical significance; n = 3 per group. Regression analysis of transcript levels indicates Pax6 and IL-1β expression are negatively correlated in Aire KO mice.