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. 2013 Oct 15;8(10):e76055. doi: 10.1371/journal.pone.0076055

Figure 2. Verification of the pluripotency of the iPSC lines with the TDP-43 A90V mutation.

Figure 2

(A) Fluorescence microscopy images of the expression of the pluripotency markers NANOG, OCT4, SSEA4, TRA-1-60, and TRA-1-81 in control (37L25) and patient (36L10) iPSC lines. Scale bar: 20 µm. (B) All iPSC lines differentiated into cells of the three germ layers, as shown by expression of desmin (mesoderm), TUJ1 (ectoderm), and alpha-fetoprotein (AFP, endoderm). These analyses indicate iPSC lines generated here are indeed pluripotent. Scale bar: 20 µm.