. Author manuscript; available in PMC: 2014 Oct 1.

Published in final edited form as: Exp Parasitol. 2013 Jul 10;135(2):274–282. doi: 10.1016/j.exppara.2013.06.017

Table 2.

Number of positives found by CAA-ELISA and CAA UCP-LF assay with respect to defined specificity cut-off thresholds.

	number of CAA positives
Total number of samples: N=1979	high specificity cut-off^a	low specificity cut-off^b
ELISA	51 (3%)	282 (14%)
UCP-LF	170 (9%)	536 (27%)

The high specificity cut-off threshold OD for the CAA-ELISA is 1,5 x OD-value of plate cutoff; plate cut-off is defined as the average OD of 4 buffers + 3 SD’s. The high specificity cut-off threshold T/FC ratio for the CAA UCP-LF assay is the maximum ratio of 86 negative samples + 2 SD’s.

The low specificity cut-off OD for the CAA-ELISA is the plate cuf-off. The low specificity T/FC ratio CAA UCP-LF assay is the average ratio + 3 SD’s).