Table 2.
number of CAA positives | ||
---|---|---|
Total number of samples: N=1979 | high specificity cut-offa | low specificity cut-offb |
ELISA | 51 (3%) | 282 (14%) |
UCP-LF | 170 (9%) | 536 (27%) |
The high specificity cut-off threshold OD for the CAA-ELISA is 1,5 x OD-value of plate cutoff; plate cut-off is defined as the average OD of 4 buffers + 3 SD’s. The high specificity cut-off threshold T/FC ratio for the CAA UCP-LF assay is the maximum ratio of 86 negative samples + 2 SD’s.
The low specificity cut-off OD for the CAA-ELISA is the plate cuf-off. The low specificity T/FC ratio CAA UCP-LF assay is the average ratio + 3 SD’s).