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Effects of insulin and IGF1 on intracellular signaling pathways. The Gsk3b−/− and Gsk3b+/+ MEF cells were treated with 10 nM insulin (A) or 50 ng/ml IGF1 (B) for the indicated time. The levels of phosphorylated and unphosphorylated proteins were determined by Western blot analysis. Equal loading of proteins was confirmed by reprobing the blot for GAPDH.
