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. Author manuscript; available in PMC: 2014 Oct 1.
Published in final edited form as: DNA Repair (Amst). 2013 Jul 27;12(10):835–843. doi: 10.1016/j.dnarep.2013.07.004

Fig. 1.

Fig. 1

Design and generation of the Rmi1hy/hy mice. (A) Scheme showing the gene trap strategy used to disrupt the Rmi1 gene. Exons (E) 1 through 3 are shown by filled boxes. The trapping cassette shows the splice acceptor (SA) the neomycin sequence (Neo) and the polyadenylation sequence (pA). Primers used for genotyping are indicated by arrows. (B) Agarose gel showing PCR products of the genotyping strategy. (C) Percentages of one month old wild-type (n=89), Rmi1wt/hy (n=146) and Rmi1hy/hy (n=0) adult mice obtained from Rmi1wt/hy intercrosses (235 total offspring analyzed). (D) Quantitative RT- PCR of Rmi1 expression in 9.5 dpc wild-type, Rmi1wt/hy and Rmi1hy/hy embryos. Primers used (qRmi1f and qRmi1r) are indicated by arrowheads in A. (E) Quantitative RT-PCR of expression of components of the BTR complex and control genes in 9.5 dpc wild-type and Rmi1hy/hy embryos. Primers used are described in supplementary Table 1.