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. Author manuscript; available in PMC: 2014 Nov 1.
Published in final edited form as: Psychoneuroendocrinology. 2013 Jun 18;38(11):10.1016/j.psyneuen.2013.05.016. doi: 10.1016/j.psyneuen.2013.05.016

Figure 5. Comparison of the distribution of 3H-8-OH-DPAT binding with that of SUMO1-5-HT1A-R.

Figure 5

Subcellular fractions (1ml/fraction) were generated by discontinuous gradient centrifugation with 10%–40% iodixanol. Each fraction was divided into two parts, one for the 3H-8-OH-DPAT binding assay and one for immunoprecipitation for SUMO1 and immunoblot for 5-HT1A-R, Gαz protein, ubc9 and markers of subcellular organelles. Top graph compares the profiles of the distribution of 3H-8-OH-DPAT binding (solid line) with that of SUMO1-5-HT1A-R (dashed line), in which IOD of the blot was measured and calculated as % of the peak fraction. Bottom panel shows immunoblots of 5-HT1A-R, Gαz protein, ubc 9 and markers of subcellular organelles. The SUMO1-5-HT1A-Rs were determined by immunoprecipitation with SUMO1 antibody followed by 5-HT1A-R.