Table 1.
Antibodies used for immunoprecipitation and immunoblots
| Antibodies | IP | Immunoblot | Source |
|---|---|---|---|
| Mouse-anti-SUMO-1 (D-11) | 4 µg | Santa Cruz #sc-5308 | |
| Rabbit-anti-SUMO-1 | 1:1,000 | Enzo #BML-PW0505 | |
| Rabbit-anti-Gαz (1–20) | 4 µg | 1:2,000 | Santa Cruz #sc-388 |
| Rabbit-anti-5HT1A receptor | 15 µg | 1:500 | Gift from Dr. Scrogin |
| Mouse-anti-Flotillin-1 | 1:2,000 | BD #610820 | |
| Rabbit-anti-Ubiquitin | 1:1,000 | DAKO#Z0458 | |
| Goat-anti-ubc9 | 1:200 | Santa Cruz #sc-5231 | |
| Mouse-anti-Na+/K+ ATPase α1 | 1:2,000 | Santa Cruz #sc-21712 | |
| Rabbit-anti-calreticulin | 1:2,000 | Abcam #Ab4 | |
| Rabbit-anti-calnexin | 1:10,000 (1 hour) | GenScript #A01240 | |
| Rabbit-anti-LMAN1 | 1:500 | Epitomic #T3357 | |
| Rabbit-anti-TGN38 (M290)* | 1:1,000 | Santa Cruz #sc-33784 | |
| HRP conjugated LDH | 1:4,000 | Abcam #Ab7639-1 | |
| Goat-anti-EEA1 (C-15) | 1:1,000 | Santa Cruz #sc-6414 | |
| Goat-anti-Lamp1 (C20) | 1:200 | Santa Cruz #sc-8098 | |
| Mouse-anti-β-actin (C4) | 1:20,000 (30–60 min) | MP #08691001 |
Abcam: Abcam Inc. Cambridge, MA; BD: BD Biosciences San Jose, CA; DAKO: DAKO A/S, Denmark; Enzo: Enzo Life Sciences Inc. Farmingdale, NY; Epitomics: Epitomics Inc. Burlingame, CA; MP: MP Biomedicals, LLC, Solon, OH; Novus: Novus Biologicals Inc., Littleton, Co; Dr. Scrogin: Karie E Scrogin at Loyola University Chicago; Santa Cruz: Santa Cruz Biotechnology Inc., Santa Cruz, CA.
*
Wash with 2% milk in 1% Tween 20 TBS.