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. Author manuscript; available in PMC: 2014 Nov 1.
Published in final edited form as: Cell Microbiol. 2013 Jul 2;15(11):1783–1795. doi: 10.1111/cmi.12157

Fig. 6. Identification of the natural substrates of VipD.

Fig. 6

Total lipids from mitochondria treated with His6-VipD or His6-VipDS73A were extracted using the Folch method. The composition of the lipids was profiled by mass spectrometry on an LTQ Orbitrap instrument. Shown are representative base peak chromatograms of mitochondrial lipid extracts obtained in positive (A) and negative (B) ion modes using reversed-phase chromatography. Lysophospholipid species with significant increase in His6-VipD treated samples detected by LC-MS/MS (C).