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Diagrams of the reference genes used and the target genes investigated in quantitative polymerase chain reaction (PCR) analyses. (A) Internal control standard curve; (B) POLD standard curve; (C) internal control solubility curve; (D) POLD solubility curve; (E) internal control amplification curves; (F) POLD amplification curves compositions and multi-interference factors. POLD1, DNA polymerase δ catalytic subunit gene 1.
