Table 1.
Comparison of the average (mean ± SD) yield and ratio of A 260nm /A 280nm of RNA Isolate using 3 different methods; LeukoLOCK™, Tempus™, and TRIzol® reagent
| Methods | LeukoLOCK™(n=10) | Tempus™(n=10) | TRIzol® reagent (n=10) |
|---|---|---|---|
| A 260nm /A 280nm | 2.06 ± 0.05 | 1.97 ± 0.06 | 1.98 ± 0.06 |
| Yield (μg/ml) | 0.99 ± 0.07 | 1.08 ± 0.09 | 3.78 ± 0.12 |
The ratios of A260nm/A280nm for the 3 RNA extracts from the 3 different methods were > 1.80, indicating high RNA purity without contamination by protein and other organic molecules.