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. 2013 Oct 1;2:494. doi: 10.1186/2193-1801-2-494

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© Kulkarni et al.; licensee Springer. 2013

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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SDS-PAGE profile of the crude lysate of the cells carrying plasmid with reverse-oriented insert (lane 1), crude lysate of the cells carrying Mi EO construct (lane 2) and the purified recombinant Mi EO protein (lane 3). Sizes of the proteins (kDa) in the molecular marker are indicated on the left side.