Table 2.
Cyanobacteria and other bacteria in the Sacramento-San Joaquin Delta and Clear Lake identified by molecular analyses
| Location | SampleID | APHN-FL | DLCH-LE | DLCH-sp | LMNC-LI | LMNR-RO | LMNR-sp | MCRC-AE | ALGR-sp | aPRTB | BCLL-PU | FLVC-TA | PNBC-AL | PNBC-sp | RHDB-SP | RHDB-sp | SYNC-sp | UNKWNa |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Clear Lake | CL3(6)b | 21 | 2 | 1 | 2 | 6 | 9 | 6 | ||||||||||
| CL4(7) | 14 | 6 | ||||||||||||||||
| S2 | 1 | 1 | 1 | 12 | 1 | 1 | 2 | |||||||||||
| S1(8) | 4 | 2 | 1 | 12 | ||||||||||||||
| CL1(9) | 20 | |||||||||||||||||
| Sacramento-San Joaquin Delta | D16(7)c | 23 | 2 | |||||||||||||||
| D19(9) | 20 | |||||||||||||||||
| D19(9) | 13 | 7 |
Numbers indicate the number of clones classified into each category.
Twenty clones were analyzed for each sample, except for CL3(6) that used 50 clones for sequencing. The sequencing reaction did not work for some of the clones, affecting the total number of clones available in this Table. The obtained 16S rDNA sequences were subjected to clustering into Operational Taxonomic Units and similarity search by BLASTN program. Phylogenetic analysis was conducted to identify closely related taxa.
Abbreviations: [cyanobacteria] APHN-FL: Aphanizomenon flos-aquae, DLCH-LE: Dolichospermum lemmermannii, DLCH-sp: Dolichospermum sp., LMNC-LI: Limnococcus limneticus, LMNR-RO: Limnoraphis robusta, LMNR-sp: Limnoraphis-sp., MCRC-AE: Microcystis aeruginosa, [other bacteria] ALGR-sp: Algoriphagus sp., aPRTB: alpha proteobacterium, BCLL-PU: Bacillus pumilus, FLVC-TA: Fluviicola taffensis, PNBC-AL: Paenibacillus alvei, PNBC-sp: Paenibacillus sp., RHDB-SP: Rhodobacter sphaeroides, RHDB-sp: Rhodobacter sp., SYNC-sp: Synechococcus sp., UNKWN: unknown bacteria
aThese two sequences were designated as unidentified bacteria (maximum identity by BLASTN search <95%).
bThe sampling month is shown in parenthesis in Sample ID.
cDifferent primer set (CYA108F and CYA16S SCYR) was used for the sample D16(7).