Table 1.
GFP |
WT-α2-chn |
G228S-α2-chn |
|||
---|---|---|---|---|---|
Before | After | Before | After | Before | After |
25.97 | 61.14* | 36.93 | 54.90* | 34.01 | 33.86 |
aPercentage of filopodia oriented within the rostroventral quadrant (Fig. 4C) between 180° and 270°, before and after the 52 hpf transition point, in neurons expressing the GFP, WT-α2-chn, or G228S-α2-chn mutant constructs (based on movies of 9, 4, and 5 neurons, respectively, for before and 10, 8, and 6 neurons after; numbers of filopodia were in the range of 70–173 per condition). This quadrant represents the area where the MR and IR muscle targets lie. Distribution was compared by two-way ANOVA. There was a significant difference in filopodial orientation between “Before” and “After” in both GFP- or WT-α2-chn-expressing neurons (p < 0.001 in both cases). There was no difference in filopodial orientation “Before” in any situation; or between “Before” and “After” in G228S-α2-chn-expressing neurons (p > 0.05). Filopodial distribution of G228S-α2-chn-expressing neurons “After” was significantly different compared with that of GFP- or WT-α2-chn-expressing neurons “After,” however (p = 0.009 and p = 0.033, respectively).
*Significant distribution difference.