Table 3.
Fast reset (Hz) |
Unilaterality |
Slow tracking movements |
||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
wt | miMO1 | MO1 | miMO2 | MO2 | wt | miMO1 | MO1 | miMO2 | MO2 | wt | miMO1 | MO1 | miMO2 | MO2 |
0.254 | 0.202 | 0.065 | 0.241 | 0.025 | 15/113 | 7/37 | 8/54 | 4/15 | 20/75 | 113/113 | 37/37 | 6/54 | 15/15 | 11/75 |
aQuantitation of OKR in wt, control morpholinos (miMO1, miMO2), and MO1- and MO2-injected fish (n = 113, n = 37, n = 54, n = 15, and n = 75, respectively) from three different experiments. Morpholino-injected fish display almost no OKR, as reflected by the number of fast reset movements observed, with a p < 0.0001 for MO1 and MO2 compared with wt, miMO1, and miMO2. The unilaterality of the response is not significantly affected, however, as a similar percentage of fish have a unilateral defect in all groups. Finally, although all wt, miMO1, and miMO2 fish are capable of slow tracking movements, only a fraction MO1- and MO2-injected fish can perform these.