Figure 1.

A, B, Whole-cell patch recording demonstrates the modulation of the frequency and waveform of action potentials by endogenous SLOB in motor neurons in vivo. Action potentials were evoked in motor neuron cell bodies under current-clamp mode. The starting voltage was adjusted to −80 mV by the injection of an appropriate amount of holding current, and action potentials were evoked by the subsequent injection of 200 pA of depolarizing current of 500 ms duration in wild-type (A) and slob null (B) flies. C, The number of action potentials evoked by different depolarizing current injections. Error bars represent SEM. Wild type, n = 10; slob, n = 5. **p < 0.005, Mann–Whitney U test. D, Superimposition of the first spikes evoked by a 200 pA current injection in motor neurons from wild-type and slob null flies.