Figure 3.

slob57 and slob71 promoters exhibit different transcriptional activity. Promoter regions upstream of the identified TSSs for slob57 and slob71 were cloned into the pGL4.10[luc2] vector to drive the luciferase (luc) reporter gene. Drosophila S2 cells were transfected with various slob promoter–luc constructs and the pCMV–LacZ vector as an internal control. The relative luciferase activity is the luciferase activity normalized to β-gal activity and is reported as the fold change compared with the empty luc control vector. A, Summary of luciferase activity experiments with slob57 promoters. B, Summary of luciferase activity experiments with slob71 promoters. Relative luciferase activity driven by slob71 promoters is higher than that of slob57. *p < 0.05, ***p < 0.001, relative to the empty luc vector, one-way ANOVA with Bonferroni's post hoc test. n ≥ 3 independent experiments. Error bars represent SEM.