Figure 4.

The DPE and MTE are important regulators of transcription from the slob71 promoter. Core nucleotides within the MTE, DPE, or both MTE and DPE were mutated in the slob71 −1966 to +81 promoter. Luciferase activity was measured in Drosophila S2 cells transfected with mutant or intact control constructs. The relative luciferase activity is the luciferase activity normalized to β-gal activity and is reported as the percentage of activity exhibited by the intact control construct. ***p < 0.001 compared with −1966 to +81, one-way ANOVA with Bonferroni's post hoc test. n = 4 independent experiments. Error bars represent SEM.