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. 2013 Oct 16;33(42):16767–16777. doi: 10.1523/JNEUROSCI.1001-13.2013

Figure 8.

Figure 8.

slob57 and slob71 promoter activity in larval glia and motor neurons. A, B, Expression of CD8::GFP (GFP) driven by slob57 (57) at the third-instar larval NMJ. Presynaptic and postsynaptic domains are labeled using antibodies against HRP and DLG, respectively (A). Representative confocal z-stacks are shown. Magnified image of CD8::GFP localization is shown in B. Note the non-overlap between CD8::GFP, HRP, and DLG. C, D, Representative confocal z-stack showing CD8::GFP under control of slob71–Gal4 (71) at the NMJ. Magnified image in D illustrates colocalization of CD8::GFP and the motor neuron marker HRP in synaptic boutons. E, F, Expression of the nuclear marker Red-Stinger (RS) under control of slob57–Gal4 in either larval salivary gland (E) or muscle cell nuclei (F). G, H, Red-Stinger driven by slob71–Gal4 in either larval salivary gland (G) or muscle cell nuclei (H). I, CD8::GFP driven by slob57–Gal4 surrounds axonal bundles and is closely associated with REPO-positive glial nuclei. J, Colocalization of REPO and Red-Stinger driven by slob57–Gal4 in a subset of glial nuclei that surround motor neuron axonal bundles. Scale bars: A, C, I, J, 20 μm; E–H, 100 μm.