FIG. 1.

Effects of type 2 diabetes on levels of ROS in PMN. (A) Alterations in the fluorescence of DCFH-DA measured by fluorimetry; (B) Mean DCFH fluorescence assessed by static cytometry;% versus control. (C) DCFH-DA fluorescence in PMNs assessed by fluorescence microscopy; nuclei: Hoechst 33342 signal (blue); ROS: DCFH-DA signal (green). **p<0.01 ***p<0.001 versus control. DCFH-DA, 2′,7′-dichlorodihydrofluorescein diacetate; PMN, polymorphonuclear leukocytes; ROS, reactive oxygen species. To see this illustration in color, the reader is referred to the web version of this article at www.liebertpub.com/ars