Table 1.
Redox Midpoints and pKa Values of Selected Thioredoxin-Like Proteins
| Protein | Motif | Redox midpoint Eo′ (mV) [Reference] | pKa of active cysteine [Reference] |
|---|---|---|---|
| Escherichia coli thioredoxin 1 | CGPC | −284 (20) | 7.1 (20) |
| E. coli thioredoxin 2 | CGPC | −221 (20) | 5.1 (20) |
| E. coli glutaredoxin 1 | CPYC | −233 (3) | N/A |
| E. coli glutaredoxin 3 | CPYC | −198 (3) | <5.5 (44) |
| Salmonella Typhimurium SrgA | CPPC | −154 (25) | 4.7 (25) |
| S. Typhimurium ScsC | CPYC | −132 [This work] | 3.4 [This work] |
| E. coli DsbG | CPYC | −129 (59) −126 (5) |
3.5 (48) |
| S. Typhimurium DsbA | CPHC | −126 (25) | 3.3 (25) |
| Homo sapiens protein disulfide isomerase | CGHCa | −110 (22) | 4.5 (35) |
| E. coli DsbA | CPHC | −124 (3) −125 [This work] |
3.5 (43) |
| S. Typhimurium DsbL | CPFC | −97 (25) | 3.8 (25) |
| E. coli DsbC | CGYC | −130 (63) | 4.3 (55) |
| Bacillus subtilis BdbD | CPSC | −75 (11) | <4.5 (11) |
a
Human protein disulfide isomerase has two CGHC motifs.