FIG. 2.
Functional analysis of supercomplex I1III2 and Complex I in detergent-solubilized BHM. Detergent dependence of (A) NADH-ubiquinone oxidoreductase activity and (B) NADH-cytochrome c oxidoreductase activity. Activity rates are expressed in μmoles of NADH·min−1·mg protein−1. (C) The production of hydrogen peroxide was measured in the presence of 1.8 μM mucidin (dashed bars) and 1.8 μM mucidin plus 4 μM Rotenone (solid bars). Values are expressed as relative fluorescence units (RFU) by DCF normalized against Complex I activity and shown as percentage values of the reference sample (i.e., control BHM in the presence of mucidin only). The corresponding percentage values of RFU·mg protein−1 in the presence of mucidin plus rotenone are 121±20 and 158±61, respectively in control and in DDM-treated samples. DDM sample treated with 2.6 g dodecylmaltoside/g of protein; n indicates the number of independent samples. Data are given as mean±S.D. p-values were calculated using the Student's t-test. DCF, 2′,7′-dichlorofluorescein.