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Sensitivity of RFLP, PCR TaqMan, HRM, and COBAS assays. The sensitivity was evaluated by mixing codon 12 or codon 13 mutated and wild-type DNA from cell lines at 100%, 50%, 25%, 10%, 5%, 2.5%, and 1% ratios
| Codon 12 | Codon 13 | ||||||
| G12D | G12V | G12C | G12S | G12A | G12R | G13D | |
| RFLP | 2.5% | 5.0% | |||||
| TaqMan PCR | <1.0% | <1.0% | <1.0% | 5.0% | 2.5% | 5.0% | 2.5% |
| HRM PCR | 5.0% | 5.0% | |||||
| COBAS | <1.0% | <1.0% | |||||
