Figure 1.

IR laser-induced temperature jump alters linear capacitance. (A) Under whole-cell voltage clamp, an uninduced SLC26a5 HEK cell was nominally stepped to the membrane potentials indicated. During the voltage step, an IR laser pulse of 20 ms duration (nominally 40% Capella laser power) was delivered via optical fiber. Regardless of the holding potential, the laser pulse induced a fixed maximal increase in C_m, ∼10% of resting C_m. Averages are given in Results section. (B) Simultaneously measured series resistance indicates a linear increase in temperature during the pulse and an exponential cooling of bath media after the pulse. (C) An increase in duration of the pulse results in a greater C_m change. The holding potential is 0 mV.